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primary mouse anti troponin t antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank primary mouse anti troponin t antibody
    Primary Mouse Anti Troponin T Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+troponin+t/anti-Troponin+T/pmc12946758-533-3-9
    Average 96 stars, based on 269 article reviews
    primary mouse anti troponin t antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: miR-21 promotes fibrogenic epithelial-to-mesenchymal transition of epicardial mesothelial cells involving Programmed Cell Death 4 and Sprouty-1.
    Article Snippet: Antibodies Rabbit anti-WT1 (0.035 mg/ml, Abcam, Cambridge, UK, ab33821), mouse anti-Islet1 (13 mg/ml, Developmental Studies Hybridoma Bank, clone 39.4D5), rabbit anti-Nkx2.5 (4 mg/ml, Santa Cruz Biotechnology, #sc-14033), mouse anti-CD45 (20 mg/ ml, BD Biosciences, clone OX-1), mouse anti-Troponin T (1.6 mg/ml, Developmental Studies Hybridoma Bank, clone CT3), mouse anti-a-SMA (11 mg/ml, Sigma-Aldrich, clone 1A4), rabbit anti-S100A4 (0.38 mg/ml, kind gift from Dr. Eugene Lukanidin, Danish Cancer Society, Copenhagen, Denmark), rabbit anti-PDCD4 (1 mg/ml, Cell Signaling, D29C6), rabbit anti-SPRY1 (0.67 mg/ml, Santa Cruz, #sc-30048), mouse antiIgG2a,k (Sigma-Aldrich, UPC-10), mouse anti-IgG1,k (SigmaAldrich, MOPC-21), rabbit anti-IgG (Santa Cruz, #sc-2027), Alexa Fluor donkey anti-mouse 555, Alexa Fluor donkey antimouse 488, Alexa Fluor donkey anti-goat 488, Alexa Fluor donkey anti-goat 488, Alexa Fluor donkey anti-rabbit 555, Alexa Fluor donkey anti-rabbit 488, (Molecular Probes, Invitrogen, DK).

    Article Title: Fine Mapping of the 1p36 Deletion Syndrome Identifies Mutation of PRDM16 as a Cause of Cardiomyopathy
    Article Snippet: Nuclei were stained with TO-PRO-3 or DAPI (Invitrogen) and sections were mounted in Prolong Gold antifade reagent (Invitrogen).

    Article Title: Fine Mapping of the 1p36 Deletion Syndrome Identifies Mutation of PRDM16 as a Cause of Cardiomyopathy
    Article Snippet: The primary antibodies used were rabbit anti-PRDM16 (Abcam) and mouse anti-Troponin T (Developmental Studies Hybridoma Bank at the University of Iowa).

    Derivative Assay:

    Article Title: Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice.
    Article Snippet: .. Chicken anti-vimentin (Abcam, Waltham, MA, US), rabbit anti-platelet derived growth factor receptor-α (Pdgfr-α) (Abclonal, Woburn, MA, US), rabbit antiα-SMA (Cell Signaling, Danvers, MA, US and ThermoFisher, Waltham, MA, US), mouse anti-troponin T (Developmental Studies Hybridoma Bank, Iowa City, IA, US), rabbit anti-BrdU (ThermoFisher), rabbit anti-Ki67 (ThermoFisher), rabbit antiphospho-histone H3 (Abcam), and rabbit anti-CD31 (Abcam) antibodies were used. ..

    Article Title: Microenvironment Stiffness Amplifies Post-ischemia Heart Regeneration in Response to Exogenous Extracellular Matrix Proteins in Neonatal Mice
    Article Snippet: .. Chicken anti-vimentin (Abcam, Waltham, MA, US), rabbit anti-platelet derived growth factor receptor-α (Pdgfr-α) (Abclonal, Woburn, MA, US), rabbit anti-α-SMA (Cell Signaling, Danvers, MA, US and ThermoFisher, Waltham, MA, US), mouse anti-troponin T (Developmental Studies Hybridoma Bank, Iowa City, IA, US), rabbit anti-BrdU (ThermoFisher), rabbit anti-Ki67 (ThermoFisher), rabbit anti-phospho-histone H3 (Abcam), and rabbit anti-CD31 (Abcam) antibodies were used. ..

    Staining:

    Article Title: Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes
    Article Snippet: Sections were thawed and rehydrated in calcium-free, magnesium-free PBS (Corning) for 5 min, permeabilized using 0.01% Triton-X (Sigma) and blocked with 10% horse serum (Sigma) in PBS for 20 min. .. Slides were stained with primary antibodies for 1 h using: mouse anti-Troponin-T (4 μg ml −1 , Developmental Studies Hybridoma Bank, CT3), mouse anti-α-actinin (sarcomeric; 1:500 dilution, Sigma, A7811), mouse anti-connexin-43 (1:500, Abcam, ab11370), rabbit anti-hK V 11.1, hERG (1:400 dilution, Alomone Labs, APC-062) or mouse anti-connexin-40 (1:100 dilution, Life Technologies, 36-4900). .. After three washes with PBS, slides were stained for 1 h with the following secondary antibodies: Goat Anti-Mouse IgG Alexa Fluor488 (2 μg ml −1 , Life Technologies, A-11029) for Troponin-T, Goat Anti-Rabbit IgG Alexa Fluor594 (2 μg ml −1 , Life Technologies, A11037) for Cx43, Cx40 and hERG, Goat Anti-Mouse IgG Alexa Fluor 647 (2 μg ml −1 , Life Technologies, A-21236) for α-actinin.

    Article Title: Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes.
    Article Snippet: Sections were thawed and rehydrated in calcium-free, magnesium-free PBS (Corning) for 5min, permeabilized using 0.01% Triton-X (Sigma) and blocked with 10% horse serum (Sigma) in PBS for 20min. .. Slides were stained with primary antibodies for 1 h using: mouse anti-Troponin-T (4mgml 1, Developmental Studies Hybridoma Bank, CT3), mouse anti-a-actinin (sarcomeric; 1:500 dilution, Sigma, A7811), mouse anti-connexin-43 (1:500, Abcam, ab11370), rabbit anti-hKV11.1, hERG (1:400 dilution, Alomone Labs, APC-062) or mouse anti-connexin-40 (1:100 dilution, Life Technologies, 36-4900). .. After three washes with PBS, slides were stained for 1 h with the following secondary antibodies: Goat Anti-Mouse IgG Alexa Fluor488 (2 mgml 1, Life Technologies, A-11029) for Troponin-T, Goat Anti-Rabbit IgG Alexa Fluor594 (2mgml 1, Life Technologies, A11037) for Cx43, Cx40 and hERG, Goat Anti-Mouse IgG Alexa Fluor 647 (2 mgml 1, Life Technologies, A-21236) for a-actinin.



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    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker <t>cTnT</t> and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.
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    Image Search Results


    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Journal: Frontiers in Immunology

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model

    doi: 10.3389/fimmu.2025.1719392

    Figure Lengend Snippet: Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Article Snippet: Fixed aCM in suspension were co-stained with MLC2a REAfinityTM conjugated with APC (1:10, Miltenyi Biotec) and cTnT REAfinityTM conjugated with FITC (1:10, Miltenyi Biotec) antibodies and incubated for 15 min at RT.

    Techniques: Activation Assay, Injection, Single Cell, MANN-WHITNEY, Staining, Marker

    Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Journal: Frontiers in Immunology

    Article Title: GJA5 and ATP1A1 perturbations recapitulate inflammation-related beat irregularities in iPSC-based atrial myocardium tissue model

    doi: 10.3389/fimmu.2025.1719392

    Figure Lengend Snippet: Fabrication of 3D condensed tissues incorporating M1 macrophages. (A) Schematic of 3D tissue formation protocol, consisting of aCM, cfb, and monocyte preculture for 3 days (d-10), tissue seeding in 96 well plates (d-7) subsequent culture and M1 maturation for 7 days (d-7 to d0) and activation of M1 (d0). (B) Brightfield image of the injection molded pillar device used for seeding tissues. (C) Annotated representative brightfield image of condensed 3D tissue consisting of aCM+cfb. 9 D) Sharp electrode recordings of single cell aCMs in 3D tissues consisting of aCM+cfb (n=17/N=4) compared to 2D monolayer of aCM for (n=31/N=3), demonstrating significant increase in upstroke velocity for 3D tissues (Mann-Whitney test). (E) IF staining for cardiac marker cTnT and cardiac tissue resident macrophage marker CX3CR1 in aCM+cfb+M1 tissue. Zoomed-in image showing area marked by white rectangle, white arrows indicate macrophages. ****P<0.0001.

    Article Snippet: Tissues were incubated with primary antibodies (cTnT REAfinity conjugated FITC (1:100, Miltenyi Biotec)) and CX3CR1 rabbit anti-human (1H14L7) (1:250, Invitrogen) or Collagen I Polyclonal Antibody (1:100, ThermoFisher Scientific) overnight at 4 °C on a plate shaker.

    Techniques: Activation Assay, Injection, Single Cell, MANN-WHITNEY, Staining, Marker